ABSTRACT
@#AIM: To investigate the protective effects of berberine on Sprague-Dawley(SD)rat retinal Müller cells with high concentration glucose <i>in vitro</i>.<p>METHODS: The retinal Müller cells of SD rats were primary cultured by enzyme digestion. The second generation of Müller cells were randomly divided into 7 groups. They were normal glucose concentration(5mmol/L glucose)group, high glucose concentration(25mmol/L glucose)group, high glucose+ berberine group(5, 10, 25, 50 and 100μmol/L). After cultured for 24h, 48h and 72h, the cell proliferative viability was measured by CCK-8 method.<p>RESULTS: After cultured for 24h, 48h and 72h, compared to the normal glucose concentration group, the absorbance of cells in the high glucose concentration group reduced significantly(All <i>P</i><0.01). Compared to the high glucose concentration group, the absorbance of cells in different concentration berberine(10, 25, 50 and 100μmol/L)groups increased significantly(All <i>P</i><0.05). It showed a dose-dependent effect. There was no statistically significant difference on the cells absorbance between high glucose+5μmol/L berberine group and high glucose group(<i>P</i>>0.05).<p>CONCLUSION: Berberine could reduce the inhibitory effect of high glucose on the proliferative viability of Müller cells to some extent. The intensity of effect was positively correlated with the berberine concentration.
ABSTRACT
OB JECTIVE: To investigate the chemical constituents from the fermented rice substrate of endophytic fungus Xylaria sp. HCCB03890. METHODS: The compounds were isolated with silica gel column chromatography and reversed-phase high performance liquid chromatography. The structures of these compounds were elucidated through MS and NMR spectroscopic analysis. The anti-microbes activities were evaluated by disk diffusion method, cytotoxic activities were determined by MTT method, and anti-HIV activities were measured by using a firefly luciferase assay system. RESULTS: Six compounds were isolated and identified as TV-acetyltyra-mine(1), schizostatin(2), piliformic acid(3), stigmast-5,22-dien-3-ol(4), dankasterone(5) and demethylincisterol(6) on the basis of spectroscopic data analyses. Compound 3 showed moderate anti-microbial activity. Compounds 4-6 exhibited cytotoxic and anti-HIV activities, especially, and compund 5 and 6 showed significant anti-HIV activities with IC50 values of 0.4 and 0.9 μg · mL, respectively. CONCLUSION: Compounds 2, 5 and 6 were for the first time isolated from Xylaria sp., moreover, 5 and 6 are for the first time found in terrestrial microbes.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the feasibility and results of application of both expanded cutaneous flap and temporoparietal fascia flap in total ear reconstruction with Medpor framework.</p><p><b>METHODS</b>The main procedure consists of two stages: Stage I-skin expansion; Stage II -auricle formation consists of orientation of Medpor implant and creation of coverage for the implant by both expanded skin flap and temporoparietal fascia flap.</p><p><b>RESULTS</b>Twenty-two ears in 22 unilateral microtia patients were constructed using Medpor implants covered with both expanded cutaneous flap and temporoparietal fascia flap over the last three years, they were accepted as pleasing by the patients.</p><p><b>CONCLUSIONS</b>Application of both expanded cutaneous flap and temporoparietal fascia flap can assure no extrusion of Medpor implant in ear reconstruction. Either more, the two layers of transferred tissues will not affect the profile details of the reconstructed ear. And because the skin covering the framework and fascia is derived from mastoid region, the appearance and profile of the reconstructed auricle is true to nature and close to that of the opposite one.</p>